Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4.
Identifieur interne : 000112 ( Main/Exploration ); précédent : 000111; suivant : 000113Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4.
Auteurs : Sharol Schmidt [États-Unis] ; Andrei Smertenko [États-Unis]Source :
- Journal of cell science [ 1477-9137 ] ; 2019.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Appareil du fuseau (métabolisme), Arabidopsis (génétique), Arabidopsis (métabolisme), Centrosome (métabolisme), Microtubules (métabolisme), Protéines associées aux microtubules (métabolisme), Protéines d'Arabidopsis (métabolisme), Tabac (génétique), Tabac (métabolisme).
- MESH :
- génétique : Agrobacterium tumefaciens, Arabidopsis, Tabac.
- métabolisme : Appareil du fuseau, Arabidopsis, Centrosome, Microtubules, Protéines associées aux microtubules, Protéines d'Arabidopsis, Tabac.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Arabidopsis Proteins, Microtubule-Associated Proteins.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Tobacco.
- metabolism : Arabidopsis, Centrosome, Microtubules, Spindle Apparatus, Tobacco.
Abstract
Here, we show that the embryophyte (land-plant)-specific protein MACERATOR4 (MACET4) binds microtubules in vitro and in vivo, promotes microtubule polymerization at sub-critical tubulin concentrations, decreases the lag phase in microtubule bulk polymerization assays, and colocalizes with microtubule nucleation sites. Furthermore, we find that MACET4 forms oligomers that induce aster formation in vitro in a manner that is similar to aster formation mediated by centrosomes and TPX2. MACET4 is expressed during cell division and accumulates at the microtubule nucleation regions of the plant-specific cytokinetic microtubule array, the phragmoplast. We found that MACET4 localizes to the preprophase band and the cortical division zone, but not the spindle. MACET4 appears as cytoplasmic foci in vivo and forms octamers in vitro Transient expression in tobacco leaf pavement cells results in labeling of shrinking plus- and minus-ends. MACET4 facilitates microtubule depolymerization by increasing the frequency of catastrophes in vivo and by suppressing rescues in vitro Microtubules formed in the presence of MACET4 in vitro are shorter, most likely due to the depletion of the free tubulin pool. Accordingly, MACET4 knockdown results in longer phragmoplasts. We conclude that the direct activity of MACET4 is in promoting microtubule nucleation.This article has an associated First Person interview with the first author of the paper.
DOI: 10.1242/jcs.232819
PubMed: 31076517
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4.</title><author><name sortKey="Schmidt, Sharol" sort="Schmidt, Sharol" uniqKey="Schmidt S" first="Sharol" last="Schmidt">Sharol Schmidt</name><affiliation wicri:level="2"><nlm:affiliation>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Smertenko, Andrei" sort="Smertenko, Andrei" uniqKey="Smertenko A" first="Andrei" last="Smertenko">Andrei Smertenko</name><affiliation wicri:level="2"><nlm:affiliation>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA andrei.smertenko@wsu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31076517</idno><idno type="pmid">31076517</idno><idno type="doi">10.1242/jcs.232819</idno><idno type="wicri:Area/Main/Corpus">000104</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000104</idno><idno type="wicri:Area/Main/Curation">000104</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000104</idno><idno type="wicri:Area/Main/Exploration">000104</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4.</title><author><name sortKey="Schmidt, Sharol" sort="Schmidt, Sharol" uniqKey="Schmidt S" first="Sharol" last="Schmidt">Sharol Schmidt</name><affiliation wicri:level="2"><nlm:affiliation>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Smertenko, Andrei" sort="Smertenko, Andrei" uniqKey="Smertenko A" first="Andrei" last="Smertenko">Andrei Smertenko</name><affiliation wicri:level="2"><nlm:affiliation>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA andrei.smertenko@wsu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author></analytic><series><title level="j">Journal of cell science</title><idno type="eISSN">1477-9137</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (metabolism)</term><term>Arabidopsis Proteins (metabolism)</term><term>Centrosome (metabolism)</term><term>Microtubule-Associated Proteins (metabolism)</term><term>Microtubules (metabolism)</term><term>Spindle Apparatus (metabolism)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Appareil du fuseau (métabolisme)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Centrosome (métabolisme)</term><term>Microtubules (métabolisme)</term><term>Protéines associées aux microtubules (métabolisme)</term><term>Protéines d'Arabidopsis (métabolisme)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arabidopsis Proteins</term><term>Microtubule-Associated Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Centrosome</term><term>Microtubules</term><term>Spindle Apparatus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Appareil du fuseau</term><term>Arabidopsis</term><term>Centrosome</term><term>Microtubules</term><term>Protéines associées aux microtubules</term><term>Protéines d'Arabidopsis</term><term>Tabac</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Here, we show that the embryophyte (land-plant)-specific protein MACERATOR4 (MACET4) binds microtubules <i>in vitro</i> and <i>in vivo</i>, promotes microtubule polymerization at sub-critical tubulin concentrations, decreases the lag phase in microtubule bulk polymerization assays, and colocalizes with microtubule nucleation sites. Furthermore, we find that MACET4 forms oligomers that induce aster formation <i>in vitro</i> in a manner that is similar to aster formation mediated by centrosomes and TPX2. MACET4 is expressed during cell division and accumulates at the microtubule nucleation regions of the plant-specific cytokinetic microtubule array, the phragmoplast. We found that MACET4 localizes to the preprophase band and the cortical division zone, but not the spindle. MACET4 appears as cytoplasmic foci <i>in vivo</i> and forms octamers <i>in vitro</i> Transient expression in tobacco leaf pavement cells results in labeling of shrinking plus- and minus-ends. MACET4 facilitates microtubule depolymerization by increasing the frequency of catastrophes <i>in vivo</i> and by suppressing rescues <i>in vitro</i> Microtubules formed in the presence of MACET4 <i>in vitro</i> are shorter, most likely due to the depletion of the free tubulin pool. Accordingly, MACET4 knockdown results in longer phragmoplasts. We conclude that the direct activity of MACET4 is in promoting microtubule nucleation.This article has an associated First Person interview with the first author of the paper.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31076517</PMID><DateCompleted><Year>2020</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>27</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1477-9137</ISSN><JournalIssue CitedMedium="Internet"><Volume>132</Volume><Issue>11</Issue><PubDate><Year>2019</Year><Month>06</Month><Day>05</Day></PubDate></JournalIssue><Title>Journal of cell science</Title><ISOAbbreviation>J Cell Sci</ISOAbbreviation></Journal><ArticleTitle>Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">jcs232819</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1242/jcs.232819</ELocationID><Abstract><AbstractText>Here, we show that the embryophyte (land-plant)-specific protein MACERATOR4 (MACET4) binds microtubules <i>in vitro</i> and <i>in vivo</i>, promotes microtubule polymerization at sub-critical tubulin concentrations, decreases the lag phase in microtubule bulk polymerization assays, and colocalizes with microtubule nucleation sites. Furthermore, we find that MACET4 forms oligomers that induce aster formation <i>in vitro</i> in a manner that is similar to aster formation mediated by centrosomes and TPX2. MACET4 is expressed during cell division and accumulates at the microtubule nucleation regions of the plant-specific cytokinetic microtubule array, the phragmoplast. We found that MACET4 localizes to the preprophase band and the cortical division zone, but not the spindle. MACET4 appears as cytoplasmic foci <i>in vivo</i> and forms octamers <i>in vitro</i> Transient expression in tobacco leaf pavement cells results in labeling of shrinking plus- and minus-ends. MACET4 facilitates microtubule depolymerization by increasing the frequency of catastrophes <i>in vivo</i> and by suppressing rescues <i>in vitro</i> Microtubules formed in the presence of MACET4 <i>in vitro</i> are shorter, most likely due to the depletion of the free tubulin pool. Accordingly, MACET4 knockdown results in longer phragmoplasts. We conclude that the direct activity of MACET4 is in promoting microtubule nucleation.This article has an associated First Person interview with the first author of the paper.</AbstractText><CopyrightInformation>© 2019. Published by The Company of Biologists Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schmidt</LastName><ForeName>Sharol</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Smertenko</LastName><ForeName>Andrei</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-5078-4881</Identifier><AffiliationInfo><Affiliation>Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA andrei.smertenko@wsu.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Cell Sci</MedlineTA><NlmUniqueID>0052457</NlmUniqueID><ISSNLinking>0021-9533</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000707922">AT1G23790 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008869">Microtubule-Associated Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018385" MajorTopicYN="N">Centrosome</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008869" MajorTopicYN="N">Microtubule-Associated Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008870" MajorTopicYN="N">Microtubules</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008941" MajorTopicYN="N">Spindle Apparatus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Cytokinesis</Keyword><Keyword MajorTopicYN="Y">Microtubules</Keyword><Keyword MajorTopicYN="Y">Nucleation</Keyword><Keyword MajorTopicYN="Y">Phragmoplast</Keyword></KeywordList><CoiStatement>Competing interestsThe authors declare no competing or financial interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>05</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31076517</ArticleId><ArticleId IdType="pii">jcs.232819</ArticleId><ArticleId IdType="doi">10.1242/jcs.232819</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Schmidt, Sharol" sort="Schmidt, Sharol" uniqKey="Schmidt S" first="Sharol" last="Schmidt">Sharol Schmidt</name></region><name sortKey="Smertenko, Andrei" sort="Smertenko, Andrei" uniqKey="Smertenko A" first="Andrei" last="Smertenko">Andrei Smertenko</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000112 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000112 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31076517
|texte= Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31076517" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |